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human primary cd3 t cells  (Miltenyi Biotec)


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    Miltenyi Biotec human primary cd3 t cells
    Human Primary Cd3 T Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+primary+cd3+t+cells/CD3+MicroBeads%2C+human+-+lyophilized/med_rxiv__2025__07__29__25332340-211-0-22
    Average 96 stars, based on 113 article reviews
    human primary cd3 t cells - by Bioz Stars, 2026-10
    96/100 stars

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    Isolation:

    Article Title: Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seq
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated with Lymphoprep density gradient medium (STEMCELL, ST-07801) and stored at -150°C in CryoStor CS10 (Sigma, C2874-100ML). .. Human primary CD3 + T cells, CD4 + T cells, and B cells were isolated from freshly thawed frozen PBMCs using MACS (Myltenyi, 130-096-535, 130-096-533 and 130-101-638, respectively). .. We sorted PBMCs into eight CD4 + T cell subsets with purity > 95% using a FACS Aria III (BD Biosciences) with the flowcytometry staining panel reported in the ImmuNexUT ( Supplementary Figure 9 and Supplementary Table 16 ).

    Article Title: 1-Pyrroline-5-carboxylate released by prostate Cancer cell inhibit T cell proliferation and function by targeting SHP1/cytochrome c oxidoreductase/ROS Axis
    Article Snippet: .. Human primary CD3 + T cells were isolated from healthy people blood using a CD3εMicroBead kit (Miltenyi Biotec Inc. CA, USA), according to the manufacturer’s instructions. .. The purity of the CD3 + T-cell preparation was assessed by flow cytometry (FACS Calibur, Becton, Dickinson and Company, Franklin Lakes, NJ, USA) using PE-anti-mouse CD3monoclonal antibody (mAb) (eBioscience, Inc., San Diego, CA, USA).

    Article Title: 1-Pyrroline-5-carboxylate inhibit T cell glycolysis in prostate cancer microenvironment by SHP1/PKM2/LDHB axis
    Article Snippet: .. Human primary CD3 + T cells were isolated from healthy people’s blood using a CD3 + Microbead kit (Miltenyi Biotec Inc. CA, USA), according to the manufacturer’s instructions. .. The purity of the CD3 + T-cell preparation was assessed by flow cytometry (FACS Calibur, Becton, Dickinson and Company, Franklin Lakes, NJ, USA) using PE-anti mouse CD3 monoclonal antibody (mAb) (eBioscience, Inc., San Diego, CA, USA).

    Magnetic Cell Separation:

    Article Title: Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seq
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated with Lymphoprep density gradient medium (STEMCELL, ST-07801) and stored at -150°C in CryoStor CS10 (Sigma, C2874-100ML). .. Human primary CD3 + T cells, CD4 + T cells, and B cells were isolated from freshly thawed frozen PBMCs using MACS (Myltenyi, 130-096-535, 130-096-533 and 130-101-638, respectively). .. We sorted PBMCs into eight CD4 + T cell subsets with purity > 95% using a FACS Aria III (BD Biosciences) with the flowcytometry staining panel reported in the ImmuNexUT ( Supplementary Figure 9 and Supplementary Table 16 ).



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